Science

Objectiv

  • Study of physical properties of synthetic lipids, binary lipid mixtures of PC, short peptides, ATPase, S-layer peptides and Human Plasma Lipoproteins by the methods of molecular acoustic, ultrasound velocimetry, densitometry, TSM, spectroscopic and fluorimetric methods.
  • Measuring apparatus software and hardware design and development.

Experiences

September 2001 - Present
Department of Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Slovakia Scientific assistant.
September 2003 - October 2003
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada - The interactions of alpha-helical transmembrane peptides with Phosphatidylcholine bilayers study by the DSC and FTIR spectroscopy.
April 2003
Institute of Physical Chemistry Polish Academy of Sciences Warszawa, Poland. DNA hybridisation study by the QCM method.
October 2000 - September 2001
Department of Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, civil service
November 1999 - October 2000
special practice in biochemistry laboratory, Institute of Biochemistry and Food Chemistry, Technical University of Graz, Graz, Austria. Investigation of human plasma lipoproteins (HDL3 and LDL) oxidation by the fluorescent methods
November 1998
Center of Ultrastructure Research of the University of Agricultural Sciences, Vienna. S-layers and hemoglobine activity study.
September 1997 - October 2000
Expert laboratory work within PGD study concerning investigation of biophysical mechanisms of oxidation of lipids and lipoproteins, Faculty of Mathematics and Physics, Comenius University, Bratislava
September - October 1996
special practice in membranological laboratory, Faculty of Mathematics and Physics, Comenius University, Bratislava study of binary lipid mixtures phase transitions on liposomes by the method of molecular acoustic and densitometry
June - July 1996
special practice in membranological laboratory, Faculty of Mathematics and Physics, Comenius University, Bratislava study of physical properties of human plasma lipoproteins (HDL3 and LDL) by the method of molecular acoustic and densitometry
June - July 1995
basic practice in membranological laboratory, Faculty of Mathematics and Physics, Comenius University, Bratislava investigation of interaction adrenocorticotropin (ACTH1-24) with unilamellar liposomes from DMPC prepared by exstruction method

Publications In Extenso

  1. Tibor Hianik, Peter Rybár, Roland Krivánek, Mária Petríková, Milena Roudna and Hans-Jürgen Apell
    Specific volume and compressibility of bilayer lipid membranes with incorporated Na,K-ATPase
    Gen. Physiol. Biophys. (2011), 30, 145–153
  2. Maja Šnejdárková, Alexandra Poturnayová, Peter Rybár, Pavel Lhoták, Michal Himl, Karolína Flídrová, Tibor Hianik
    High sensitive calixarene-based sensor for detection of dopamine by electrochemical and acoustic methods.
    Bioelectrochemistry 80 (2010) 55–61
  3. Svobodova L, Snejdarkova M, Polohova V, Grman I, Rybar P, Hianik T.
    QCM immunosensor based on polyamidoamine dedrimers.
    Electroanalysis 18 (2006) 1943-1949
  4. Hianik T, Rybar P, Andreev SY, Oretskaya TS, Vadgama P
    Detection of DNA hybridization on a liposome surface using ultrasound velocimetry and turbidimetry methods 
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 14 (15): 3897-3900 AUG 2 2004
  5. McElhaney RN, Krivanek R, Rybar P, Lewis RNAH, Liu F, Hodges RS
    On the minimal hydrophobicity required for helical peptides to maintain stable transmembrane associations with lipid bilayers 
    BIOPHYSICAL JOURNAL 86 (1): 376A-376A Part 2 Suppl. S JAN 2004
  6. Hianik T, Rybar P, Benediktyova Z, Svobodova L, Hermetter A
    Effects of oxidation on changes of compressibility of bovine serum albumin 
    GENERAL PHYSIOLOGY AND BIOPHYSICS 22 (4): 467-476 DEC 2003
  7. Hianik T, Rybar P, Sikurova L, Hermetter A
    Changes of compressibility of low density lipoproteins following copper mediated oxidation 
    GENERAL PHYSIOLOGY AND BIOPHYSICS 22 (2): 139-149 JUN 2003
  8. Krivanek R, Rybar P, Kupcu S, Sleytr UB, Hianik T
    Affinity interactions on a liposome surface detected by ultrasound velocimetry 
    BIOELECTROCHEMISTRY 55 (1-2): 57-59 JAN 2002
  9. Hianik T, Rybar P, Svobodova L, Kresak S, Nikolelis DP
    Liposome aggregation in presence of the sweeteners cyclamate and saccharine 
    PHARMAZIE 56 (8): 633-635 AUG 2001
  10. Krivanek R, Rybar P, Prenner EJ, McElhaney RN, Hianik T
    Interaction of the antimicrobial peptide gramicidin S with dimyristoyl-phosphatidylcholine bilayer membranes: a densitometry and sound velocimetry study 
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1510 (1-2): 452-463 FEB 9 2001
  11. Hianik T, Rybar P, Bernhardt I
    Adiabatic compressibility of red blood cell membrane: influence of skeleton 
    BIOELECTROCHEMISTRY 52 (2): 197-201 DEC 2000
  12. Hianik T, Rybar P, Sikurova L, Dujsik J, Krivanek R, Chorvat D, Markin VS, Landers DF
    Local anesthetic tetracaine influences the physical properties of globular proteins 
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 174 (3): 341-348 DEC 1 2000
  13. Hianik T, Rybar P, Fajkus M, Krivanek R, Sikurova L, Markin VS, Landers DF
    The local anesthetic tetracaine changes physical properties of BLM, liposomes, and globular proteins. 
    BIOPHYSICAL JOURNAL 76 (1): A272-A272 Part 2 JAN 1999
  14. Hianik T, Kupcu S, Sleytr UB, Rybar P, Krivanek R, Kaatze U
    Interaction of crystalline bacterial cell surface proteins with lipid bilayers in liposomes. A sound velocity study 
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 147 (3): 331-339 FEB 15 1999
  15. Hianik T, Rybar P, Kostner GM, Hermetter A
    Molecular acoustic as a new tool for the study of biophysical properties of lipoproteins 
    BIOPHYSICAL CHEMISTRY 67 (1-3): 221-228 SEP 1 1997

Abstracts

  1. Hianik T., Rybár P., Vitoviè P., Kresák S., Samuely T., Krivánek R., Urban J., Stefaneková E., Lewis R. N. A. H., McElhaney R. N.: The Mechanism of Interaction of Model alpha-Helical Peptides with Lipid Bilayers and Monolayers, XVII International Symposium on Bioelectrochemistry and Bioenergetics, Florence, Italy, June 19-24, Book of Abstracts (2003)
  2. Krivánek R., Kuepcue S., Rybár P., Sleytr U. B., Hianik T.: Affinity Interactions on a Liposome Surface Detected by Ultrasound Velocimetry, XVI International Symposium on Bioelectrochemistry and Bioenergetics, Bratislava, June 1-6 2001, Book of Abstracts p. 89 (2001)
  3. Hianik T., Rybár P., Bernhardt I.: Adiabatic compressibility of red blood cell membrane influence skeleton, poster, 12th Meeting of the European Association for Red Cell Research, April 15-19, Book of Abstracts (1999) (Otzenhausen, Germany)
  4. Hianik T., Rybár P., Fajkus M., Krivánek R., ©ikurová L., Markin V. S., Landers D. F.: The local anesthetic tetracaine changes physical properties of BLM, liposomes, and globular proteins. Biophysical Journal 76 (1999) A272-A272 Part 2
  5. Hianik T., Krivánek R., Rybár P.: Targeted Immobilization of Immunoglobulins on Lipid Bilayers Through Bacterial Proteins, Book of Abstracts 5th Workshop: Manufacturing of Laboratory Prototypes, Otrokovice 8-10.10. (1999) Czech Republic

Theses

  1. P. Rybar: Oxidacia lipoproteinov krvnej plazmy, vplyv antioxidantov, RNDr theses, Januar 2000 (in slovak language)
  2. P. Rybar: Štúdium vplyvu oxidačných procesov na fyzikálne vlastnosti biokoloidov, PhD theses, September 2003 (in slovak language)